For the introduction part of the step of the harvest process of the incubated culture medium it should
be noted that the work under sterile conditions and with non-contaminated material was the most
important fact of all but unfortunately it was far from easy to have these high standard conditions
during the whole production process and through all of the production stages because the used
equipment was mostly self-prepared with very simple materials.
This is also the reason for this relatively impure result of the Penicillin Sodium Salt after the last step of production. However, the research team with
the mastermind of Lord Howard W. Florey, Ernst B. Chain, Norman G. Heatley, et.al. did excellent
work to find an extraction method for the massive production of Penicillin and with this huge
investigation work they were able to save more than 82,000,000 lives, up until the present day.
The used equipment for the harvest
process was a changing trolley with 4 wooden slabs and 4 rectangular porcelain culture vessels on it. The volume of one batch is divided into the volume of 4 culture vessels what fit with the volume of both the collecting bottle and the filtration bottle what is all in all a little bit less than 1 gallon. The culture medium will be drawn off with the help of a special device called changing pistol. The pistol has two glass tubes and works with pressure and suction controlled by a metal trigger. The pistol is working with the refill and the replacement technique means to draw off incubated medium and to replace it with fresh one. This refresh cycle can be repeated for about 14 times at 7 day intervals until the fungus stops to produce Penicillium spores.